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KMID : 1005220120270010051
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Journal of Embryo Transfer
2012 Volume.27 No. 1 p.51 ~ p.55
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The Effects of Cryoprotectants on Motility and Viability Kinetics of Liquid Boar Semen at 4℃
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Jeon Yu-Byeol
Park Kwang-Woo
Kwak Seong-Sung
Cheong Seung-A
Yoon Jun-Chul
Hyun Sang-Hwan
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Abstract
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The objective of this study was to investigate the motility and kinematics of boar sperm that while stored at 4C. The samples of fresh boar semen were place into an extender, Androhep, and stored at 4℃. In three of these samples, cryoprotectants were added. The sperm's motilities and kinematics were evaluated by using microscope (×400) and the viability status was evaluated by using with eosin staining method. The 5 sample groups are; Goup A:Androhep (extender), stored at 17℃. Group B:Androhep (extender), stored at 4℃. Group C:Androhep (extender), + 3% glycerol (cryoprotectant), stored at 4℃. Group D:Androhep (extender), + 3% DMSO (cryoprotectant), stored at 4℃. Group E: Androhep (extender), + 3% ethylene glycol (cryoprotectant), stored at 4℃. In group A, the sperm's motility was reduced. On day one the sperm's motility was (85.7 ± 2.3) and day 5 the motility was (43.9 ± 3.3). In group B, C and D the sperm's motility were reduced to 0 on day 5. In group E the sperm's percentage of motility decreased. On day one the sperm's motility was (42.0 ± 0.5) and day 5 the motility was (2.3 ± 0.3). When comparing cryoprotectant in samples of boar sperm there is a slight improvement in the results when the use of Androhep Lite (extender), + 3% ethylene glycol (cryoprotectant), stored at 4℃ are used. Based on these results, ethylene glycol can protect sperm from heat shock at 4℃, but not satisfactory level. However, it showed the possibilities of liquid semen preservation at 4℃ by using cryoprotectant.
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KEYWORD
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4℃ , boar semen , storage time , cryoprotectant , heat shock
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